Voltage-dependent L-type calci

Mouse Voltage- dependent L- type calcium channel subunit beta- 1, CACNB1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CACNB1,CAB1; CACNLB1; CCHLB1; calcium channel voltage-dependent subunit beta 1; calcium channel, L type, beta 1 polypeptide; dihydropyridine-sensitive L-type, calcium channel beta-1 subunit; voltage-dependent L-type calcium channel subunit beta-1; calcium channel, voltage-dependent, beta 1 subunit

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Voltage- dependent L- type calcium channel subunit beta- 1, CACNB1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CACNB1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CACNB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.