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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Golgi apparatus protein 1, Glg1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Golgi apparatus protein 1, GLG1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GLG1,CFR-1; ESL-1; MG-160; MG160; E-selectin ligand 1; cysteine-rich fibroblast growth factor receptor; golgi sialoglycoprotein MG-160; golgi glycoprotein 1, Golgi apparatus protein 1
Search name
Mouse GLG1 ELISA KIT , Mouse GLG ELISA KIT,Mouse CFR-1 ELISA KIT ,Mouse ESL-1 ELISA KIT ,Mouse MG-160 ELISA KIT ,Mouse MG160 ELISA KIT ,Mouse E-selectin ligand 1 ELISA KIT ,Mouse cysteine-rich fibroblast growth factor receptor ELISA KIT ,Mouse golgi sialoglycoprotein MG-160 ELISA KIT ,Mouse golgi glycoprotein 1 ELISA KIT ,Mouse Golgi apparatus protein 1 ELISA KIT, Mouse Golgi apparatus protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Golgi apparatus protein 1 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi apparatus protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGolgi apparatus protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Golgi apparatus protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Golgi apparatus protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Synchronizing Protein Transport in the Secretory Pathway
or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the Golgi apparatus, where they are processed and sorted toward their final destinations along the secretory pathway. It is now clear that many Golgi
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
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