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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
HEAT repeat-containing protein 3, Heatr3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse HEAT repeat- containing protein 3, Heatr3 ELISA KIT
Product Name:Mouse HEAT repeat- containing protein 3, Heatr3 ELISA KIT
Packing:96T
Catalog No.:ELI-48172m
Gene Name:Mouse Heatr3
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Heatr3
Alternative Name:Mouse HEAT repeat-containing protein 3, Heatr3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse HEAT repeat- containing protein 3, Heatr3 ELISA KIT allows for the in vitro quantitative determination of Mouse Heatr3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse HEAT repeat- containing protein 3, Heatr3 ELISA KIT has been pre-coated with an Mouse HEAT repeat-containing protein 3, Heatr3 antibody specific to Mouse Heatr3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Heatr3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Heatr3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Heatr3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
. 21. Incubate for 30 min at room temperature. Enrichment of Ligation Fragments Containing the Protein of Interest in the Complex 22. Remove 10 µL from the sample (from Step 21). Store at -20°C. This sample will serve
. The mouse should be wrapped in a tissue to keep it warm (loss of body heat is common in abdominal surgery) or alternatively placed on a heat pad, and allowed to recover. Animals which are placed under anesthetic should always be supervised and monitored
Procedure to purify protein from cells
This procedure is used to break cells that contain a protein to be purified.This procedure is valid only for the use on Appling's lab sonicatior. PREPARATION 1.Place your sample in a 15ml elongated plastic tube such that the sample-tube wall
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