DENN domain-containing protein

Mouse DENN domain- containing protein 4B, Dennd4b ELISA KIT

Product Name:Mouse DENN domain- containing protein 4B, Dennd4b ELISA KIT
Packing:96T

Catalog No.:ELI-47004m

Gene Name:Mouse Dennd4b

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Dennd4b

Alternative Name:Mouse DENN domain-containing protein 4B, Dennd4b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse DENN domain- containing protein 4B, Dennd4b ELISA KIT allows for the in vitro quantitative determination of Mouse Dennd4b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse DENN domain- containing protein 4B, Dennd4b ELISA KIT has been pre-coated with an Mouse DENN domain-containing protein 4B, Dennd4b antibody specific to Mouse Dennd4b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dennd4b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dennd4b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dennd4b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!