DNA polymerase epsilon subunit
4, Pole4 ELISA KIT/ Mouse DNA polymerase epsilon subunit 4, Pole4 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-46907m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
DNA polymerase epsilon subunit 4, Pole4
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse DNA polymerase epsilon subunit 4, Pole4 ELISA KIT
Product Name:Mouse DNA polymerase epsilon subunit 4, Pole4 ELISA KIT
Packing:96T
Catalog No.:ELI-46907m
Gene Name:Mouse Pole4
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Pole4
Alternative Name:Mouse DNA polymerase epsilon subunit 4, Pole4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA polymerase epsilon subunit 4, Pole4 ELISA KIT allows for the in vitro quantitative determination of Mouse Pole4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA polymerase epsilon subunit 4, Pole4 ELISA KIT has been pre-coated with an Mouse DNA polymerase epsilon subunit 4, Pole4 antibody specific to Mouse Pole4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pole4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pole4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pole4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications
immunosorbent assay (PCR-ELISA). The antibody was first immobilized on antigen-coated microplates; in a second step, a commercially available DNA-labeled species-specific antibody was added; and finally the deoxyribonucleic marker was amplified in a PCR step
Avoiding DNA Contamination in RT-PCR
Figure 3 . DNA Contamination in RNA. Mouse liver total RNA was isolated according to protocol. RT-PCR reactions were performed using Ambion's RETROscript?Kit and 0.5 µg RNA. PCR reactions were performed with 5 µg RNA. 10 µl of each reaction
for selection of stable clones. Alternative selection markers can be chosen from various antibiotic resistance genes or fluorescent markers such as enhanced green fluorescent protein (EGFP). Prepare plasmid DNA (~ 1 µg/µL) using a QIAGEN miniprep kit
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