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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Keratin, type II cuticular Hb3, Krt83
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Keratin, type II cuticular Hb3, Krt83 ELISA KIT
Product Name:Mouse Keratin, type II cuticular Hb3, Krt83 ELISA KIT
Packing:96T
Catalog No.:ELI-46528m
Gene Name:Mouse Krt83
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Krt83
Alternative Name:Mouse Keratin, type II cuticular Hb3, Krt83
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Keratin, type II cuticular Hb3, Krt83 ELISA KIT allows for the in vitro quantitative determination of Mouse Krt83 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Keratin, type II cuticular Hb3, Krt83 ELISA KIT has been pre-coated with an Mouse Keratin, type II cuticular Hb3, Krt83 antibody specific to Mouse Krt83 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Krt83 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Krt83 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Krt83 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Summary The intermediate filament (IF) cytoskeleton of mammalian epithelia is generated from pairs of type I and type II keratins that are encoded by two large gene families, made up of 54 genes in humans and the mouse
and enzymes that harbors the ability torecognize and cleave foreign nucleic acids based on their sequencesignature. Three known CRISPR systems, types I, II, and III, havebeen described so far [ 12 – 15 ]. Among these different CRISPRsystems, type II CRISPR
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Normal mouse serum (NMS, Sigma) AEC kit (Vector Laboratories) Hematoxylin (Sigma
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