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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Polycomb group RING finger protein 6, Pcgf6
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Polycomb group RING finger protein 6, Pcgf6 ELISA KIT
Product Name:Mouse Polycomb group RING finger protein 6, Pcgf6 ELISA KIT
Packing:96T
Catalog No.:ELI-45086m
Gene Name:Mouse Pcgf6
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Pcgf6
Alternative Name:Mouse Polycomb group RING finger protein 6, Pcgf6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Polycomb group RING finger protein 6, Pcgf6 ELISA KIT allows for the in vitro quantitative determination of Mouse Pcgf6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Polycomb group RING finger protein 6, Pcgf6 ELISA KIT has been pre-coated with an Mouse Polycomb group RING finger protein 6, Pcgf6 antibody specific to Mouse Pcgf6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pcgf6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pcgf6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pcgf6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
strongly to chromatin (for example, the Polycomb group proteins), or lamin. However, some other nuclear proteins might have a more labile nuclear localization and might not resist to that procedure (for example, components of the RNAi machinery
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
sapiens folate receptor 1 (adult) (FOLR1), transcript variant 2, mRNA 514 NM_004924 Homo sapiens actinin, alpha 4 (ACTN4), mRNA 1187 NM_002931 Homo sapiens ring finger protein 1 (RING1), mRNA 576 NM_000020 Homo sapiens activin A receptor type II
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