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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ubiquitin-protein ligase E3C, Ube3c
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT
Product Name:Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT
Packing:96T
Catalog No.:ELI-44764m
Gene Name:Mouse Ube3c
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ube3c
Alternative Name:Mouse Ubiquitin-protein ligase E3C, Ube3c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT allows for the in vitro quantitative determination of Mouse Ube3c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- protein ligase E3C, Ube3c ELISA KIT has been pre-coated with an Mouse Ubiquitin-protein ligase E3C, Ube3c antibody specific to Mouse Ube3c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ube3c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ube3c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ube3c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
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