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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Putative DNA repair and recombination protein RAD26-like,
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Putative DNA repair and recombination protein RAD26- like, RAD26L ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ERCC6L2,RAD26L; C9orf102; SR278; excision repair cross-complementing rodent repair deficiency, complementation group 6-like 2; stretch responsive protein 278; excision repair cross-complementation group 6-like 2
Search name
Mouse ERCC6L2 ELISA KIT ,Mouse RAD26L ELISA KIT ,Mouse C9orf102 ELISA KIT ,Mouse SR278 ELISA KIT ,Mouse excision repair cross-complementing rodent repair deficiency ELISA KIT ,Mouse complementation group 6-like 2 ELISA KIT ,Mouse stretch responsive protein 278 ELISA KIT ,Mouse excision repair cross-complementation group 6-like 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Putative DNA repair and recombination protein RAD26- like, RAD26L concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD26L, During the reaction, RAD26L in the sample or standard competes with a fixed amount of biotin-labeled RAD26L for sites on a pre-coated Monoclonal antibody specific to RAD26L. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RAD26L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
Homologous recombination (HR) is a high-fidelity DNA repair pathway that maintains genome integrity, by repairing double strand breaks (DSBs) and single-stranded DNA (ssDNA) gaps and by supporting stalled/collapsed replication forks. The RecA
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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