Purine nucleoside phosphorylas

Mouse Purine nucleoside phosphorylase, PNP ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PNP,NP,Pnp1,nucleoside phosphorylase,purine nucleoside phosphorylase; PRO1837; PUNP; HEL-S-156an; epididymis secretory sperm binding protein Li 156an; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase

Search name

Mouse PNP ELISA KIT ,Mouse NP ELISA KIT ,Mouse Pnp1 ELISA KIT ,Mouse nucleoside phosphorylase ELISA KIT ,Mouse purine nucleoside phosphorylase ELISA KIT ,Mouse PRO1837 ELISA KIT ,Mouse PUNP ELISA KIT ,Mouse HEL-S-156an ELISA KIT ,Mouse epididymis secretory sperm binding protein Li 156an ELISA KIT ,Mouse inosine phosphorylase ELISA KIT ,Mouse inosine-guanosine phosphorylase ELISA KIT ,Mouse purine-nucleoside:orthophosphate ribosyltransferase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Purine nucleoside phosphorylase, PNP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PNP, During the reaction, PNP in the sample or standard competes with a fixed amount of biotin-labeled PNP for sites on a pre-coated Monoclonal antibody specific to PNP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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