Fibroblast growth factor 3, Fg

Mouse Fibroblast growth factor 3, FGF3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

FGF3,Heparin-binding growth factor 3(HBGF-3),Proto-oncogene Int-2, FGF-3,HBGF-3; INT2; FGF-3; INT-2 proto-oncogene protein; V-INT2 murine mammary tumor virus integration site oncogene homolog; fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); heparin-binding growth factor 3; murine mammary tumor virus integration site 2, mouse; oncogene INT2; proto-oncogene Int-2; fibroblast growth factor 3

Search name

Mouse FGF3 ELISA KIT ,Mouse Heparin-binding growth factor 3 ELISA KIT ,Mouse HBGF-3 ELISA KIT ,Mouse Proto-oncogene Int-2 ELISA KIT ,Mouse  FGF-3 ELISA KIT ,Mouse HBGF-3 ELISA KIT ,Mouse INT2 ELISA KIT ,Mouse FGF-3 ELISA KIT ,Mouse INT-2 proto-oncogene protein ELISA KIT ,Mouse V-INT2 murine mammary tumor virus integration site oncogene homolog ELISA KIT ,Mouse heparin-binding growth factor 3 ELISA KIT ,Mouse murine mammary tumor virus integration site 2 ELISA KIT ,Mouse oncogene INT2 ELISA KIT ,Mouse proto-oncogene Int-2 ELISA KIT ,Mouse fibroblast growth factor 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse fibroblast growth factor 3,FGF-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FGF-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGF-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGF-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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