NADP-dependent malic enzyme, M
e1 ELISA KIT/ Mouse NADP-dependent malic enzyme, Me1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-43023m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
NADP-dependent malic enzyme, Me1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse NADP- dependent malic enzyme, Me1 ELISA KIT
Product Name:Mouse NADP- dependent malic enzyme, Me1 ELISA KIT
Packing:96T
Catalog No.:ELI-43023m
Gene Name:Mouse Me1
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Me1
Alternative Name:Mouse NADP-dependent malic enzyme, Me1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse NADP- dependent malic enzyme, Me1 ELISA KIT allows for the in vitro quantitative determination of Mouse Me1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse NADP- dependent malic enzyme, Me1 ELISA KIT has been pre-coated with an Mouse NADP-dependent malic enzyme, Me1 antibody specific to Mouse Me1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Me1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Me1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Me1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验为催化苹果酸脱羧反应的酶。正式名称称为苹果酸脱氢酶（脱羧作用）。已知有三种苹果酸酶（ EC1． 1． 1． 38— 40）。其中以 NADP为受体的酶（ EC1． 1． 1． 40）催化生成下列反应： Δ G°′ =-0.36千卡。丙酮酸羧化反应（是 H． G． Wood和 C． H． Werkman， 1938最初发现的反应）中有的是苹果酸酶的作用，有的是丙酮酸羧化酶的作用，在生物体内已知以后者的作用为主。两者都是供给三羧酸循环中间体的补充反应
Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
反应。 (三)C4途径的反应过程 C4途径中的反应虽因植物种类不同而有差异，但基本上可分为羧化、还原或转氨、脱羧和底物再生四个阶段。 图4-22 叶片的横切面图，表明了C3和C4植物形态解剖的差异。 上左C4单子叶植物（甘蔗）；中左C3单子叶（一种草）；上右C4双子叶植物（Asteracea）。中右C4植物叶片的三维模式图 根据植物所形成的C4二羧酸的种类以及脱羧反应参与的酶类，又可把C4途径分为三种亚类型：①依赖NADP的苹果酸酶(NADP malic enzyme)的苹果酸型(NADP
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