Monocarboxylate transporter 1,

Mouse Monocarboxylate transporter 1, MCT 1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Monocarboxylate transporter 1,MCT 1,Solute carrier family 16 member 1, SLC16A1,MCT1, HHF7; MCT; MCT 1; solute carrier family 16 monocarboxylic acid transporters member 1; solute carrier family 16 member 1; solute carrier family 16 member 1 monocarboxylic acid transporter 1; solute carrier family 16 monocarboxylate transporter member 1

Search name

Mouse Monocarboxylate transporter 1 ELISA KIT ,Mouse SLC16A1 ELISA KIT,Mouse MCT1 ELISA KIT ,Mouse Solute carrier family 16 member 1 ELISA KIT , Mouse HHF7 ELISA KIT ,Mouse MCT ELISA KIT ,Mouse MCT 1 ELISA KIT ,Mouse solute carrier family 16 monocarboxylic acid transporters member 1 ELISA KIT ,Mouse solute carrier family 16 member 1 ELISA KIT ,Mouse solute carrier family 16 member 1 monocarboxylic acid transporter 1 ELISA KIT ,Mouse solute carrier family 16 monocarboxylate transporter member 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse monocarboxylate transporter 1,MCT 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCT 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCT 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCT 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MCT 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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