Heat shock protein beta-9, Hsp
b9 ELISA KIT/ Mouse Heat shock protein beta-9, Hspb9 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-42624m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Heat shock protein beta-9, Hspb9
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Heat shock protein beta- 9, Hspb9 ELISA KIT
Product Name:Mouse Heat shock protein beta- 9, Hspb9 ELISA KIT
Packing:96T
Catalog No.:ELI-42624m
Gene Name:Mouse Hspb9
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Hspb9
Alternative Name:Mouse Heat shock protein beta-9, Hspb9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Heat shock protein beta- 9, Hspb9 ELISA KIT allows for the in vitro quantitative determination of Mouse Hspb9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Heat shock protein beta- 9, Hspb9 ELISA KIT has been pre-coated with an Mouse Heat shock protein beta-9, Hspb9 antibody specific to Mouse Hspb9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hspb9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hspb9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hspb9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolation of Heat Shock Protein Complexes
Heat shock proteins (Hsp) are molecular chaperones with the capability to interact with a wide range of other proteins and are thus often found coupled with other heat shock and non-heat shock proteins. This can be an advantage to study
指将生物的整体、组织、细胞等从其生活的温度范围内急剧地从低温移向高温时，可显著地促进合成的一组蛋白质。例如将果蝇的幼虫或培养细胞从28℃移至 35℃时，则几乎大部分的蛋白质合成停止；与此相反，而休克蛋白的合成却反而被促进。这种促进作用主要是在转录 DNA的合成（转录）阶段产生的。同样的现象也见于哺乳类动物、培养细胞、原生动物、植物组织和细菌等。另外观察到，由休克以外的其他处理也会发生类似的现象。这种现象的生理意义尚不清楚，但推测是与生物的温度适应现象有关系。
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
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