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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Trace amine-associated receptor 7d, Taar7d
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Trace amine- associated receptor 7d, Taar7d ELISA KIT
Product Name:Mouse Trace amine- associated receptor 7d, Taar7d ELISA KIT
Packing:96T
Catalog No.:ELI-41679m
Gene Name:Mouse Taar7d
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Taar7d
Alternative Name:Mouse Trace amine-associated receptor 7d, Taar7d
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Trace amine- associated receptor 7d, Taar7d ELISA KIT allows for the in vitro quantitative determination of Mouse Taar7d concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Trace amine- associated receptor 7d, Taar7d ELISA KIT has been pre-coated with an Mouse Trace amine-associated receptor 7d, Taar7d antibody specific to Mouse Taar7d .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Taar7d and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Taar7d , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Taar7d in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling
It is evident that G protein-coupled receptors (GPCRs) such as D2 dopamine receptor and functionally related Trace Amine Associated Receptor 1 (TAAR1) can engage both in G protein-dependent (e.g., cAMP-mediated) and -independent β-arrestin
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
extraction (TRI Reagent®), a filter-binding based extraction (RNAqueous®), by centrifugation through a CsCl cushion, and by two rounds of oligo d(T) selection using Ambion's Poly(A)Pure™ Kit (see Figure 1a). Regardless of whether reverse transcriptase
Preparation of probes for in situ hybridization
. Labeling is then conducted for 24-48 h in the dark, at room temperature, with occasional vortexing. NB: Any trace of free amine, such as Tris base, should be removed from the nucleic acid, as it will also react with the fluorophore.
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