Mouse Serine/ threonine-protein kinase SIK2, SIK2 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 2.7.11.1,Serine/threonine-protein kinase SIK2,Serine/threonine-protein kinase SNF1-like kinase 2,Qin-induced kinase,Salt-inducible protein kinase 2,SIK-2, SIK2,serine/threonine-protein kinase SIK2; LOH11CR1I; QIK; SNF1LK2; SIK-2; SNF1-like kinase 2; qin-induced kinase; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SNF1-like kinase 2; salt-inducible kinase 2
Search name
Mouse Class 2.7.11.1 ELISA KIT ,Mouse Serine/threonine-protein kinase SIK2 ELISA KIT ,Mouse Serine/threonine-protein kinase SNF1-like kinase 2 ELISA KIT ,Mouse Qin-induced kinase ELISA KIT ,Mouse Salt-inducible protein kinase 2 ELISA KIT ,Mouse SIK-2 ELISA KIT ,Mouse SIK2 ELISA KIT ,Mouse serine/threonine-protein kinase SIK2 ELISA KIT ,Mouse LOH11CR1I ELISA KIT ,Mouse QIK ELISA KIT ,Mouse SNF1LK2 ELISA KIT ,Mouse SIK-2 ELISA KIT ,Mouse SNF1-like kinase 2 ELISA KIT ,Mouse qin-induced kinase ELISA KIT ,Mouse salt-inducible protein kinase 2 ELISA KIT ,Mouse salt-inducible serine/threonine kinase 2 ELISA KIT ,Mouse serine/threonine-protein kinase SNF1-like kinase 2 ELISA KIT ,Mouse salt-inducible kinase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Serine/threonine-protein kinase SIK2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase SIK2, During the reaction, Serine/threonine-protein kinase SIK2 in the sample or standard competes with a fixed amount of biotin-labeled Serine/threonine-protein kinase SIK2 for sites on a pre-coated Monoclonal antibody specific to Serine/threonine-protein kinase SIK2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serine/threonine-protein kinase SIK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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