Rap1 GTPase-activating protein
2, Rap1gap2 ELISA KIT/ Mouse Rap1 GTPase-activating protein 2, Rap1gap2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-41235m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Rap1 GTPase-activating protein 2, Rap1gap2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Rap1 GTPase- activating protein 2, Rap1gap2 ELISA KIT
Product Name:Mouse Rap1 GTPase- activating protein 2, Rap1gap2 ELISA KIT
Packing:96T
Catalog No.:ELI-41235m
Gene Name:Mouse Rap1gap2
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Rap1gap2
Alternative Name:Mouse Rap1 GTPase-activating protein 2, Rap1gap2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Rap1 GTPase- activating protein 2, Rap1gap2 ELISA KIT allows for the in vitro quantitative determination of Mouse Rap1gap2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Rap1 GTPase- activating protein 2, Rap1gap2 ELISA KIT has been pre-coated with an Mouse Rap1 GTPase-activating protein 2, Rap1gap2 antibody specific to Mouse Rap1gap2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rap1gap2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rap1gap2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rap1gap2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Fluorescence Microscopy Study of Rap1 Subcellular Localization
The Ras-related GTPase Rap has been implicated in multiple cellular functions. In particular, Rap1 is a crucial regulator of both inside-out integrin activation and outside-in E-cadherin-mediated signaling. Thus, Rap1
结合结构域（Raf RBD）与GFP的突变种YFP和CFP进行融合构建。他们将Ras和YFP融合，RafRBD与CFP融合，当两分子靠的足够近时，它们之间就会激发FRET，设计的蛋白Raichu-Ras， Raichu代表和Ras结合的嵌合单元。当把Raichu-Ras与特异性的GEFs（guanine-nucleotide exchange factors）和GAPs（GTPase-activating proteins）共表达，清楚的显示FRET的增加和减少与Ras突变体的激活和抑制
激发FRET,设计的蛋白Raichu-Ras,Raichu代表和Ras结合的嵌合单元。当把Raichu-Ras与特异性的GEFs（guanine-nucleotide exchange factors）和GAPs（GTPase-activating proteins）共表达，清楚的显示FRET的增加和减少与Ras突变体的激活和抑制有关。此外他们还用同样原理观测了Rap1激活。
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