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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Neural Wiskott-Aldrich syndrome protein, Wasl
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Neural Wiskott- Aldrich syndrome protein, Wasl ELISA KIT
Product Name:Mouse Neural Wiskott- Aldrich syndrome protein, Wasl ELISA KIT
Packing:96T
Catalog No.:ELI-40223m
Gene Name:Mouse Wasl
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Wasl
Alternative Name:Mouse Neural Wiskott-Aldrich syndrome protein, Wasl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Neural Wiskott- Aldrich syndrome protein, Wasl ELISA KIT allows for the in vitro quantitative determination of Mouse Wasl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Neural Wiskott- Aldrich syndrome protein, Wasl ELISA KIT has been pre-coated with an Mouse Neural Wiskott-Aldrich syndrome protein, Wasl antibody specific to Mouse Wasl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Wasl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Wasl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Wasl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses cell proliferation in cultures, starting with primary cell cultures isolated from the mouse subventricular zone (SVZ), in which SVZ?derived NSC
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
8‐Isoprostane EIA kit (8‐iso‐PGF 2α ; Cayman Chemical) containing: Precoated (mouse anti‐rabbit IgG) EIA 96‐well strip plates
Keywords: human embryonic stem cells; mouse neural stem cells; MTT assay; 96?well plate assays; toxicology GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online
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