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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Thioredoxin-like protein 4B, Txnl4b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Thioredoxin- like protein 4B, Txnl4b ELISA KIT
Product Name:Mouse Thioredoxin- like protein 4B, Txnl4b ELISA KIT
Packing:96T
Catalog No.:ELI-40214m
Gene Name:Mouse Txnl4b
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Txnl4b
Alternative Name:Mouse Thioredoxin-like protein 4B, Txnl4b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Thioredoxin- like protein 4B, Txnl4b ELISA KIT allows for the in vitro quantitative determination of Mouse Txnl4b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Thioredoxin- like protein 4B, Txnl4b ELISA KIT has been pre-coated with an Mouse Thioredoxin-like protein 4B, Txnl4b antibody specific to Mouse Txnl4b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Txnl4b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Txnl4b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Txnl4b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
:
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文献和实验蛋白质翻译后修饰 (Protein translational modifications,PTMs) 通过功能基团或蛋白质的共价添加、调节亚基的蛋白水解切割或整个蛋白质的降解来增加蛋白质组的功能多样性。这些修饰包括磷酸化、糖基化、泛素化、亚硝基化、甲基化、乙酰化、脂质化和蛋白水解,几乎影响正常细胞生物学和发病机制的所有方面。因此,识别和理解 PTM 在细胞生物学和疾病治疗和预防的研究中至关重要。 看到一篇 Thermo Fisher的文章,关于翻译后修饰Post
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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