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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Vacuolar protein sorting-associated protein 35, Vps35 ELI
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse VPS35 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse VPS35 ELISA KIT ,Mouse VPS35 retromer complex component ELISA KIT ,Mouse MEM3 ELISA KIT ,Mouse PARK17 ELISA KIT ,Mouse vacuolar protein sorting-associated protein 35 ELISA KIT ,Mouse hVPS35 ELISA KIT ,Mouse maternal-embryonic 3 ELISA KIT ,Mouse vacuolar protein sorting 35 homolog ELISA KIT
Intended use
Mouse VPS35 ELISA kit allows for the in vitro quantitative determination of Mouse VPS35 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse VPS35 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VPS35 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VPS35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VPS35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse VPS35 ELISA kit Range 78-5000pg/ml
VPS35 Related Products
> VPS35 ELISA KITS
Bovine Vacuolar protein sorting- associated protein 35, VPS35 ELISA KIT
Human Vacuolar protein sorting- associated protein 35, VPS35 ELISA KIT
Mouse Vacuolar protein sorting- associated protein 35, Vps35 ELISA KIT
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文献和实验Screening Chemical Libraries for Compounds That Affect Protein Sorting to the Yeast Vacuole
Protein trafficking to the yeast vacuole has been extensively studied using a series of deletion mutants. In these genetic screens, mis-targeted vacuolar cargo proteins were used as phenotype markers. Here we describe a similar approach
, which leads to the isolation of mutants and to the identification of proteins involved in protein quality control and ER-associated degradation (ERAD). The method resides in ethylmethane sulfonate (EMS) mutagenesis of a yeast strain followed by screening
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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