Tetratricopeptide repeat prote
in 23, Ttc23 ELISA KIT/ Mouse Tetratricopeptide repeat protein 23, Ttc23 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-40380m
- 2025年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Tetratricopeptide repeat protein 23, Ttc23
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Tetratricopeptide repeat protein 23, Ttc23 ELISA KIT
Product Name:Mouse Tetratricopeptide repeat protein 23, Ttc23 ELISA KIT
Packing:96T
Catalog No.:ELI-40380m
Gene Name:Mouse Ttc23
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ttc23
Alternative Name:Mouse Tetratricopeptide repeat protein 23, Ttc23
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tetratricopeptide repeat protein 23, Ttc23 ELISA KIT allows for the in vitro quantitative determination of Mouse Ttc23 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Tetratricopeptide repeat protein 23, Ttc23 ELISA KIT has been pre-coated with an Mouse Tetratricopeptide repeat protein 23, Ttc23 antibody specific to Mouse Ttc23 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ttc23 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ttc23 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ttc23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
：
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
for selection of stable clones. Alternative selection markers can be chosen from various antibiotic resistance genes or fluorescent markers such as enhanced green fluorescent protein (EGFP). Prepare plasmid DNA (~ 1 µg/µL) using a QIAGEN miniprep kit
the membrane 3 x 10 min 3 x 5 min Incubate with second antibody 1 hr 30 min Wash the membrane 3 x 10 min 3 x 5 min Add substrate 5 min
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