U6 snRNA-associated Sm-like pr
otein LSm3, Lsm3 ELISA KIT/ Mouse U6 snRNA-associated Sm-like protein LSm3, Lsm3 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-39796m
- 2026年01月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
U6 snRNA-associated Sm-like protein LSm3, Lsm3
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse U6 snRNA- associated Sm- like protein LSm3, Lsm3 ELISA KIT
Product Name:Mouse U6 snRNA- associated Sm- like protein LSm3, Lsm3 ELISA KIT
Packing:96T
Catalog No.:ELI-39796m
Gene Name:Mouse Lsm3
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Lsm3
Alternative Name:Mouse U6 snRNA-associated Sm-like protein LSm3, Lsm3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse U6 snRNA- associated Sm- like protein LSm3, Lsm3 ELISA KIT allows for the in vitro quantitative determination of Mouse Lsm3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse U6 snRNA- associated Sm- like protein LSm3, Lsm3 ELISA KIT has been pre-coated with an Mouse U6 snRNA-associated Sm-like protein LSm3, Lsm3 antibody specific to Mouse Lsm3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lsm3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lsm3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lsm3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验vectors, which enables the co-expression ofSpCas9 protein driven by the potent constitutive promoterCBh and sgRNA driven by the RNA Pol III human U6 promoterin mammalian cells (Fig. 3 ). CBh promoter is a hybridpromoter derived from the CAG promoter
尔化学奖。[2]近期的研究发现核糖核酸酶P也具有一些新的功能，[3]例如人类细胞核 中的核糖核酸酶P对于正常并有效地转录多种非编码RNA(包括tRNA、5S rRNA、SRP RNA和U6 snRNA)的基因是必要的，[4]这些基因是由RNA聚合酶III(人类细胞中三类主要的RNA聚合酶之一)来进行转录。 细菌 中 在细菌中，如大肠杆菌，核糖核酸酶 P具有两个组分：一条RNA链(称为M1-RNA)和一个蛋白质(称为C5蛋白)。[5][6]在细胞内，这两个组分对于功能的发挥都是必要
，用U6 snRNA表达核酶与HBV共转染，也可有效切割靶RNA. 国内体外合成核酶，并对鸭HBV S基因体外转录物定点切割，发现对病毒抗原表达的抑制作用. Welch et al使用三个发夹状核酶，在Huh7细胞中与HBV共表达，使其HBV表达下降66%，对核酶修饰后则抑制率达83%. 在真核细胞表达的成功，预示对HBV基因治疗是有效的[14-15]. 和文超et al针对HBsAg、HBcAg、HBeAg设计了5种锤头型核酶，不仅在细胞外，而且在细胞内观察到了其对HBV的抑制，各抗原及病毒
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