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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
H-2 class II histocompatibility antigen, E-B beta chain,
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse H- 2 class II histocompatibility antigen, E- B beta chain, H2-Eb1 ELISA KIT(CUSTOM)
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
H2-Eb1,H-2Eb; H2Eb; Ia-4; Ia4; H-2 class II histocompatibility antigen, I-E beta chain; I-E beta MHC class II; MHC H2-IE-beta cell surface glycoprotein; MHC class II IE-b; cell surface glycoprotein; major histocompatibility complex H-2E beta chain; histocompatibility 2, class II antigen E beta,H-2 class II histocompatibility antigen, I-A beta chain
Search name
Mouse H2-Eb1 ELISA KIT ,Mouse H-2Eb ELISA KIT ,Mouse H2Eb ELISA KIT ,Mouse Ia-4 ELISA KIT ,Mouse Ia4 ELISA KIT ,Mouse H-2 class II histocompatibility antigen ELISA KIT ,Mouse I-E beta chain ELISA KIT ,Mouse I-E beta MHC class II ELISA KIT ,Mouse MHC H2-IE-beta cell surface glycoprotein ELISA KIT ,Mouse MHC class II IE-b ELISA KIT ,Mouse cell surface glycoprotein ELISA KIT ,Mouse major histocompatibility complex H-2E beta chain ELISA KIT ,Mouse histocompatibility 2 class II antigen E beta ELISA KIT ,Mouse H-2 class II histocompatibility antigen I-A beta chain ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse H- 2 class II histocompatibility antigen, E- B beta chain, H2-Eb1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2-Eb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for H2-Eb1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain H2-Eb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of H2-Eb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
:
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文献和实验Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes
The ability of B lymphocytes to capture external antigens (Ag) and present them as peptide fragments, loaded on Major Histocompatibility complex (MHC) class II molecules, to CD4+ T cells is a crucial part of the adaptive immune response
Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA
Major histocompatibility class I and II (MHC-I and MHC-II) antigens (Ags), as well as a variety of cell surface receptors, have been shown to enter the endocytic pathway of antigen-presenting cells (APCs). MHC Ags endocytose into clathrin
to result predominately from a human anti-mouse major histocompatibility complex (MHC) class II reactivity. Our recent development of NOD-scid IL2rγ null β2m null and NOD-scid IL2rγ null Ab null stocks of mice now make it possible to investigate
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