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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Non-specific lipid-transfer protein, Scp2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Non- specific lipid- transfer protein, Scp2 ELISA KIT
Product Name:Mouse Non- specific lipid- transfer protein, Scp2 ELISA KIT
Packing:96T
Catalog No.:ELI-39623m
Gene Name:Mouse Scp2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Scp2
Alternative Name:Mouse Non-specific lipid-transfer protein, Scp2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Non- specific lipid- transfer protein, Scp2 ELISA KIT allows for the in vitro quantitative determination of Mouse Scp2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Non- specific lipid- transfer protein, Scp2 ELISA KIT has been pre-coated with an Mouse Non-specific lipid-transfer protein, Scp2 antibody specific to Mouse Scp2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Scp2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Scp2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Scp2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验,500 rpm). Collect supernatant and centrifuge at 100,000 x g for 1 hour (Ti 45, 40,000 rpm). Filter the supernatant fraction through cheesecloth to remove lipid. 3. Dialyze the filtered supe against 10 l of cold water for 4 hours, followed
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