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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Myoblast determination protein 1, Myod1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Myoblast determination protein 1, MYOD1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MYOD1,MyoD,MYF3; PUM; bHLHc1; class C basic helix-loop-helix protein 1; myf-3; myoblast determination protein 1; myogenic factor 3; myogenic differentiation 1,Class C basic helix-loop-helix protein 1(bHLHc1);Myogenic factor 3(Myf-3)
Search name
Mouse MYOD1 ELISA KIT ,Mouse MyoD ELISA KIT ,Mouse MYF3 ELISA KIT ,Mouse PUM ELISA KIT ,Mouse bHLHc1 ELISA KIT ,Mouse class C basic helix-loop-helix protein 1 ELISA KIT ,Mouse myf-3 ELISA KIT ,Mouse myoblast determination protein 1 ELISA KIT ,Mouse myogenic factor 3 ELISA KIT ,Mouse myogenic differentiation 1 ELISA KIT ,Mouse Class C basic helix-loop-helix protein 1 ELISA KIT ,Mouse bHLHc1;Myogenic factor 3 ELISA KIT ,Mouse Myf-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Myoblast determination protein 1, MYOD1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYOD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MYOD1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MYOD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MYOD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Methods to Detect Protein Glutathionylation
of Protein Glutathionylation by Mass Spectrometry Basic Protocol 3: Determination of Global Cellular Protein Glutathionylation by Immunoblotting Alternate Protocol 1: Detecting the Glutathionylation of a Specific
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Contents: Table of Contents Adhesion Protein Protocols Preface Contents Contributors 1— Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules Selective Protocols of Immunizati... 1— Introduction 2— Materials 3— Methods 4—
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