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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
NFAT activation molecule 1, Nfam1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse NFAT activation molecule 1, NFAM1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NFAT activation molecule 1,Calcineurin/NFAT-activating ITAM-containing protein,NFAT-activating protein with ITAM motif 1, NFAM1,CNAIP,bK126B4.4; NFAT activation molecule 1; NFAT-activating protein with ITAM motif 1; calcinerin/NFAT-activating ITAM-containing protein; calcineurin/NFAT-activating ITAM-containing protein; NFAT activating protein with ITAM motif 1
Search name
Mouse NFAT activation molecule 1 ELISA KIT ,Mouse Calcineurin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse NFAT-activating protein with ITAM motif 1 ELISA KIT ,Mouse NFAM1 ELISA KIT ,Mouse CNAIP ELISA KIT ,Mouse bK126B4.4 ELISA KIT ,Mouse NFAT activation molecule 1 ELISA KIT ,Mouse NFAT-activating protein with ITAM motif 1 ELISA KIT ,Mouse calcinerin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse calcineurin/NFAT-activating ITAM-containing protein ELISA KIT ,Mouse NFAT activating protein with ITAM motif 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse nfat activation molecule 1,NFAM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFAM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NFAM1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NFAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NFAM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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