Phosphatidylethanolamine N-met
hyltransferase, Pemt ELISA / Mouse Phosphatidylethanolamine N-methyltransferase, Pemt ELISA- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-37735m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Phosphatidylethanolamine N-methyltransferase, Pemt ELISA
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Phosphatidylethanolamine N-methyltransferase,PEMT ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse Pemt ELISA KIT ,Mouse phosphatidylethanolamine N methyltransferase ELISA KIT ,Mouse PEAMT ELISA KIT ,Mouse PEMPT ELISA KIT ,Mouse PEMT2 ELISA KIT ,Mouse PNMT ELISA KIT ,Mouse phosphatidylethanolamine N-methyltransferase ELISA KIT
Intended use
Mouse PEMT ELISA kit allows for the in vitro quantitative determination of Mouse Phosphatidylethanolamine N-methyltransferase,PEMT concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PEMT, During the reaction, PEMT in the sample or standard competes with a fixed amount of biotin-labeled PEMT for sites on a pre-coated Monoclonal antibody specific to PEMT. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PEMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Performing Bronchoalveolar Lavage in the Mouse
lavage. Vet. Clin. Small Anim. 33:69‐88. Blé, F.‐X, Cannet, C., Zurbruegg, S., Karmouty Quintana, H., Frossard, N., Trifilieff, A., and Beckmann, N. 2008. Allergen‐induced lung
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
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