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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytosolic phospholipase A2 epsilon, Pla2g4e
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e ELISA KIT
Product Name:Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e ELISA KIT
Packing:96T
Catalog No.:ELI-37654m
Gene Name:Mouse Pla2g4e
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pla2g4e
Alternative Name:Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e ELISA KIT allows for the in vitro quantitative determination of Mouse Pla2g4e concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e ELISA KIT has been pre-coated with an Mouse Cytosolic phospholipase A2 epsilon, Pla2g4e antibody specific to Mouse Pla2g4e .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pla2g4e and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pla2g4e , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pla2g4e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
antiserum specific for the C terminus of epsilon isoform, protease digestion of intact 14-3-3 showed that the N-terminal half of 14-3-3 (a 16 kDa fragment) was an intact, dimeric domain of the protein. Two isoforms of 14-3-3, tau and epsilon, were expressed
Introduction of human 14-3-3 proteins
-3-3 proteins are cytosolic,a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes.Using a panel of isoform-specific antisera we find that the epsilon,eta,gamma,beta,and zeta isoforms are all present
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