Ig heavy chain V region VH558
A1/A4, Gm5629 ELISA KIT/ Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-37573m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ig heavy chain V region VH558 A1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 ELISA KIT
Product Name:Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 ELISA KIT
Packing:96T
Catalog No.:ELI-37573m
Gene Name:Mouse Gm5629
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Gm5629
Alternative Name:Mouse Ig heavy chain V region VH558 A1/A4, Gm5629
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 ELISA KIT allows for the in vitro quantitative determination of Mouse Gm5629 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 ELISA KIT has been pre-coated with an Mouse Ig heavy chain V region VH558 A1/A4, Gm5629 antibody specific to Mouse Gm5629 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gm5629 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gm5629 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gm5629 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Single Cell Analysis of Synovial Tissue B-Cells
immunoglobulin (Ig) genes amplified, cloned and sequenced. The combination of the V(D)J gene segments and the pattern of somatic mutations in the V-region genes, allows us to identify clonal relationships between the isolated B cells. Once Ig genes for a heavy
;236:799 2.Flanagan JG,et al.Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing γ,ε and α genes. Nature,1982;300:709 3.Hood L,et al.T cell antigen receptor
(λ)，同一个天然Ig分子上L链的型总是相同的。正常人血清中的κ：λ约为2：1。 2．重链（heavy chain,H链） 重链大小约为轻链的2倍，含450～550个氨基酸残基，分子量约为55或75kD。每条H链含有4～5个链内二硫键所组成的环肽。不同的H链由于氨基酸组成的排列顺序、二硫键的数目和们置、含的种类和数量不同，其抗原性也不相同，根据H链抗原性的差异可将其分为5类：μ链、γ链、α链、δ链和ε链，不同H链与L链（κ或λ链）组成完整Ig的分子分别称之为IgM、IgG、IgA、IgD
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