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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transcriptional repressor NF-X1, Nfx1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transcriptional repressor NF- X1, Nfx1 ELISA KIT
Product Name:Mouse Transcriptional repressor NF- X1, Nfx1 ELISA KIT
Packing:96T
Catalog No.:ELI-36510m
Gene Name:Mouse Nfx1
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Nfx1
Alternative Name:Mouse Transcriptional repressor NF-X1, Nfx1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transcriptional repressor NF- X1, Nfx1 ELISA KIT allows for the in vitro quantitative determination of Mouse Nfx1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transcriptional repressor NF- X1, Nfx1 ELISA KIT has been pre-coated with an Mouse Transcriptional repressor NF-X1, Nfx1 antibody specific to Mouse Nfx1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nfx1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nfx1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nfx1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
‐buffered serum, calcium‐ and magnesium‐free (CMF‐PBS) 0.1% (v/v) Triton X‐100 solution in CMF‐PBS Mouse anti‐Oct4 monoclonal
Transgenic Mouse and Transgenic Rat Outline
sequences in not required for BAC transgenes. Transgene constructs are then quantitated and microinjected into (C57BL/6 X SJL)F2 mouse eggs and surgically transferred to recipients. This is provided on a fee for service basis by the Transgenic Facility
. Kim, J., Chu, J., Shen, X., Wang, J., and Orkin, S.H. 2008. An extended transcriptional network for pluripotency of embryonic stem cells. Cell 132:1049‐1061.
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