Mouse Zona pellucida sperm-binding protein 3, ZP3 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse ZP3 ELISA KIT ,Mouse zona pellucida glycoprotein ZP3 ELISA KIT ,Mouse ZP3A ELISA KIT ,Mouse ZP3B ELISA KIT ,Mouse ZPC ELISA KIT ,Mouse Zp-3 ELISA KIT ,Mouse ZP3A/ZP3B ELISA KIT ,Mouse sperm receptor ELISA KIT ,Mouse zona pellucida glycoprotein 3A (sperm receptor) ELISA KIT ,Mouse zona pellucida glycoprotein 3B ELISA KIT ,Mouse zona pellucida protein C ELISA KIT ,Mouse zona pellucida glycoprotein 3 (sperm receptor) ELISA KIT
Intended use
Mouse ZP3 ELISA kit allows for the in vitro quantitative determination of Mouse ZP3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse ZP3 ELISA kit has been pre-coated with an antibody specific to ZP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ZP3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ZP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ZP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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