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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
CD9 antigen, Cd9
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CD9 antigen, Cd9 ELISA KIT
Product Name:Mouse CD9 antigen, Cd9 ELISA KIT
Packing:96T
Catalog No.:ELI-34174m
Gene Name:Mouse Cd9
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cd9
Alternative Name:Mouse CD9 antigen, Cd9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse CD9 antigen, Cd9 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse CD9 antigen, Cd9 ELISA KIT has been pre-coated with an Mouse CD9 antigen, Cd9 antibody specific to Mouse Cd9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验样本,上样,做 WB。 超离法由于 BCA 测定的浓度数值低,用了最大上样量 25 ul,试剂盒法用的体积少一些,换算后每孔的蛋白总量如下: 样本名 蛋白总量 ug/孔 UC-1 4.50 UC-2 3.50 SXX-1 25.00 SXX-2 25.00 UXX-1 25.00 UXX-2 25.00 选用外泌体标志物 CD9 和 TSG101 作为本次的检测指标,实验结果如下: 1、在 CD9 指标检测中,超离法的两个样本 UC-1 和 UC-2 尽管每孔
thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
Tracking Analysis),该技术已被外泌体研究领域认可为外泌体表征手段之一。NTA 技术的样本处理简单、能保证外泌体原始状态、检测速度快,检测后能提供外泌体粒径和浓度信息。 图五 外泌体 NTA 图 Western Blot 分子标志物检测: WB 可以用来鉴定外泌体的标志蛋白是否存在于样本中。外泌体标志蛋白包括四跨膜蛋白家族,如 CD9、CD63 和 CD81;细胞质蛋白,如肌动蛋白(Actin)和钙磷脂结合蛋白(Annexins);参与生物功能的分子,如凋亡转接基因 2 互作蛋白 X
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