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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
B-lymphocyte antigen CD20, Ms4a1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse B- lymphocyte antigen CD20, MS4A1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MS4A1, CD20,Bp35; CVID5; LEU-16; CD20 antigen; CD20 receptor; B-lymphocyte antigen CD20,Membrane-spanning 4-domains subfamily A member 1;Leukocyte surface antigen Leu-16;B-lymphocyte surface antigen B1
Search name
Mouse MS4A1 ELISA KIT ,Mouse CD20 ELISA KIT ,Mouse Bp35 ELISA KIT ,Mouse CVID5 ELISA KIT ,Mouse LEU-16 ELISA KIT ,Mouse CD20 antigen ELISA KIT ,Mouse CD20 receptor ELISA KIT ,Mouse B-lymphocyte antigen CD20 ELISA KIT ,Mouse Membrane-spanning 4-domains subfamily A member 1 ELISA KIT; Mouse Leukocyte surface antigen Leu-16 ELISA KIT; Mouse B-lymphocyte surface antigen B1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse B- lymphocyte antigen CD20, MS4A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse B-lymphocyte antigen CD20,MS4A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse B-lymphocyte antigen CD20,MS4A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse B-lymphocyte antigen CD20,MS4A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse B-lymphocyte antigen CD20,MS4A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验The Cytotoxic T Lymphocyte Assay for Evaluating Cell-Mediated Immune Function
Evaluation of cell-mediated immunity (CMI) is a significant component in any assessment designed to predict the full range of potential immunotoxic risk underlying health risks. Among measures of CMI, the cytotoxic T Lymphocyte (CTL) response
Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells Using Flow Cytometry
for the complement component 3 (C3) cleavage fragments C3d(g) and iC3b. Additionally, CR1 is a receptor for C3b and regulates complement convertase activity. CR1 and CR2 have various functions including antigen retention by FDC, regulation of surface complement
是通过直接或间接地影响到该细胞的bcl-2的表达或功能,进而抑制细胞生长,诱导细胞凋亡,最终得以有效地挟制和杀灭肿瘤,这为抗体可用于联合化疗或降低病人耐药的临床治疗提供了有力的证据。【参考文献】[1]Golay JT,Clark EA,Beverley PC,et al. The CD20 (BP35)antigen is involved in activation of B cell from the G0 to the G1 phase of the cell cycle. J Immunol
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