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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Gap junction alpha-10 protein, Gja10
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT
Product Name:Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT
Packing:96T
Catalog No.:ELI-33591m
Gene Name:Mouse Gja10
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Gja10
Alternative Name:Mouse Gap junction alpha-10 protein, Gja10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT allows for the in vitro quantitative determination of Mouse Gja10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Gap junction alpha- 10 protein, Gja10 ELISA KIT has been pre-coated with an Mouse Gap junction alpha-10 protein, Gja10 antibody specific to Mouse Gja10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gja10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gja10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gja10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
. The transgene should contain unique markers so that its presence can be easily detected in mouse tissue DNA samples and so that its expression can be assayed and distinguished from endogenous gene expression. Sequencing of junction fragments should be carried
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Quantal Analysis of Endplate Potentials in Mouse Flexor Digitorum Brevis Muscle
and its constituent muscle fibers are short (Curr. Protoc. Mouse Biol. 1:429?444 © 2011 by John Wiley & Sons, Inc. Keywords: neuromuscular junction; endplate potential; intracellular recording; electrophysiology; quantal analysis
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