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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Catenin alpha-2, Ctnna2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Catenin alpha- 2, Ctnna2 ELISA KIT
Product Name:Mouse Catenin alpha- 2, Ctnna2 ELISA KIT
Packing:96T
Catalog No.:ELI-33573m
Gene Name:Mouse Ctnna2
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ctnna2
Alternative Name:Mouse Catenin alpha-2, Ctnna2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Catenin alpha- 2, Ctnna2 ELISA KIT allows for the in vitro quantitative determination of Mouse Ctnna2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Catenin alpha- 2, Ctnna2 ELISA KIT has been pre-coated with an Mouse Catenin alpha-2, Ctnna2 antibody specific to Mouse Ctnna2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ctnna2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ctnna2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ctnna2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
-19]; xChIP:用来研究DNA及低结合力蛋白,采用甲醛或紫外线进行 DNA 和蛋白交联,超声波片段化染色质,然后进行片段富集及后续分析,适用于多数非组蛋白的蛋白,例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后,对 DNA 进行 PCR 扩增,验证目标序列的存在。除验证实验外,ChIP DNA也可以进行测序分析,这种方法被称为 ChIP-seq [22] ;也可做芯片分析,这种方法被称为 ChIP on CHIP 或 ChIP
位升高),不知道我的这个结果是否可以说明gsk活性降低了?还是一定需要第九位的结果才能解释这个问题?似乎也有些人说216特异性不强,没有第九位有说服力,不是很能理解这其中的原因,再次感谢你! ylhuang0502 As for the GSK phosphorylation, it is clear now that, GSK serine phosphorylation (S9 for beta and S21 for alpha
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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