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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Brain protein 44, Brp44
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Brain protein 44, Brp44 ELISA KIT
Product Name:Mouse Brain protein 44, Brp44 ELISA KIT
Packing:96T
Catalog No.:ELI-33420m
Gene Name:Mouse Brp44
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Brp44
Alternative Name:Mouse Brain protein 44, Brp44
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Brain protein 44, Brp44 ELISA KIT allows for the in vitro quantitative determination of Mouse Brp44 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Brain protein 44, Brp44 ELISA KIT has been pre-coated with an Mouse Brain protein 44, Brp44 antibody specific to Mouse Brp44 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Brp44 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Brp44 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Brp44 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Protein Expression in Brain Tissue by ELISA
The enzyme-linked immunosorbent assay (ELISA) technique offers a sensitive, simple, and versatile method for quantifying as little as 100 pg of target protein in mixed cell or tissue lysates. A further advantage to the protocol is the ability
, aspirate off buffer until the settled resin:buffer ratio is 3:1 (vol:vol). Pour the column. Mix the resin gently until it is evenly suspended in the buffer, then rapidly pour the slurry into an empty 44 mm x 250 mm liquid chromatography
Porcine brain tubulin preparation
is 3:1 (vol:vol). 12. Pour the column. 13. Mix the resin gently until it is evenly suspended in the buffer, then rapidly pour the slurry into an empty 44 mm x 250 mm liquid chromatography column (clamped to a support in the cold room
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