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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
ADP-ribosylation factor 4, Arf4
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse ADP- ribosylation factor 4, Arf4 ELISA KIT
Product Name:Mouse ADP- ribosylation factor 4, Arf4 ELISA KIT
Packing:96T
Catalog No.:ELI-34523m
Gene Name:Mouse Arf4
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Arf4
Alternative Name:Mouse ADP-ribosylation factor 4, Arf4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse ADP- ribosylation factor 4, Arf4 ELISA KIT allows for the in vitro quantitative determination of Mouse Arf4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse ADP- ribosylation factor 4, Arf4 ELISA KIT has been pre-coated with an Mouse ADP-ribosylation factor 4, Arf4 antibody specific to Mouse Arf4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Arf4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Arf4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Arf4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Preparation of Myristoylated Arf1 and Arf6 Proteins
ADP-ribosylation factor (Arf) proteins were first identified as cofactors for cholera toxin-catalyzed ADP-ribosylation of the heterotrimeric G-protein Gs. Subsequent cloning led to the discovery that Arfs were part of a group of GTP-binding
ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
TheADP-ribosylation factor proteins (ARFs) are ~20-kDa guanine-nucleotidebinding proteins found in all eukaryotic cells, which are a critical part of the minimum machinery required for vesicle formation at the Golgi and other membranes (1 –
) M26066 2.8 +16 ADP-ribosylation factor 4 (ARF4) M36341.1 1.5 +116 Isoleucil-tRNA synthetase U04953
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