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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Amine oxidase [flavin-containing] B, Maob
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Amine oxidase [flavin- containing] B, MAOB ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAOB,MAO brain; MAO platelet; MAO-B; adrenalin oxidase; monoamine oxidase type B; tyramine oxidase; monoamine oxidase B, Amine oxidase [flavin-containing] B
Search name
Mouse MAOB ELISA KIT ,Mouse MAO brain ELISA KIT ,Mouse MAO platelet ELISA KIT ,Mouse MAO-B ELISA KIT ,Mouse adrenalin oxidase ELISA KIT ,Mouse monoamine oxidase type B ELISA KIT ,Mouse tyramine oxidase ELISA KIT ,Mouse monoamine oxidase B ELISA KIT ,Mouse Amine oxidase [flavin-containing] B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse amine oxidase [flavin-containing] b, MAOB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAOB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAOB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAOB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAOB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
work, my colleagues and I concluded that D-amino-acid oxidase is not present in the mouse liver (13 ). This paper examines in greater detail the methods used in reaching that conclusion. 4 ). Organs, tissues, or tissue culture cells
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
Performing Bronchoalveolar Lavage in the Mouse
Delayre‐Orthez, C., Becker, J., de Blay, F., Frossard, N., and Pons, F. 2005b. Exposure to endotoxins during sensitization prevents further endotoxin‐induced exacerbation of airway inflammation in a mouse model of allergic asthma. Int. Arch
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