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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
CCAAT
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CCAAT/enhancer- binding protein gamma, Cebpg ELISA KIT
Product Name:Mouse CCAAT/enhancer- binding protein gamma, Cebpg ELISA KIT
Packing:96T
Catalog No.:ELI-33147m
Gene Name:Mouse Cebpg
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Cebpg
Alternative Name:Mouse CCAAT/enhancer-binding protein gamma, Cebpg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse CCAAT/enhancer- binding protein gamma, Cebpg ELISA KIT allows for the in vitro quantitative determination of Mouse Cebpg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse CCAAT/enhancer- binding protein gamma, Cebpg ELISA KIT has been pre-coated with an Mouse CCAAT/enhancer-binding protein gamma, Cebpg antibody specific to Mouse Cebpg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cebpg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cebpg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cebpg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
µl (15) Add 60 µl of Protein G agarose (50% slurry, preblocked, from EZ ChIP kit) into each chromatin sample. (16) Incubate for 1 hour at 4°C with horizontal rocking. This step removes non-specific binding activities
members, including fibrillarin and nucleolin, involved in ribosomal biogenesis, histones, fibroblast growth factor, interleukin enhancer-binding factor 3 the SRC kinase adapter protein Sam68, STAT1, and EWS. Analysis of protein arginine methylation
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