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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cysteine-rich PDZ-binding protein, Cript
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cysteine- rich PDZ- binding protein, Cript ELISA KIT
Product Name:Mouse Cysteine- rich PDZ- binding protein, Cript ELISA KIT
Packing:96T
Catalog No.:ELI-32339m
Gene Name:Mouse Cript
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Cript
Alternative Name:Mouse Cysteine-rich PDZ-binding protein, Cript
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cysteine- rich PDZ- binding protein, Cript ELISA KIT allows for the in vitro quantitative determination of Mouse Cript concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cysteine- rich PDZ- binding protein, Cript ELISA KIT has been pre-coated with an Mouse Cysteine-rich PDZ-binding protein, Cript antibody specific to Mouse Cript .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cript and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cript , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cript in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Fluorescent Labeling of Specific Cysteine Residues Using CyMPL
and Labeling), which enables specific labeling by incorporating a cysteine of interest into a minimal binding site for group 12 metal ions (e.g., Cd2+ and Zn2+ ). These sites can be inserted into any region of known secondary structure in virtually any protein
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Isolation of RNA from Difficult Tissues
. Therefore, care should be taken during the initial preparation of the homogenate to ensure intact total RNA. Brain and Plant Tissues: Protein and Lipid-rich Tissues Isolating total RNA from rat or mouse brain can be very rewarding
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