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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Putative deoxyribose-phosphate aldolase, Dera
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Putative deoxyribose- phosphate aldolase, Dera ELISA KIT
Product Name:Mouse Putative deoxyribose- phosphate aldolase, Dera ELISA KIT
Packing:96T
Catalog No.:ELI-32041m
Gene Name:Mouse Dera
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Dera
Alternative Name:Mouse Putative deoxyribose-phosphate aldolase, Dera
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Putative deoxyribose- phosphate aldolase, Dera ELISA KIT allows for the in vitro quantitative determination of Mouse Dera concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Putative deoxyribose- phosphate aldolase, Dera ELISA KIT has been pre-coated with an Mouse Putative deoxyribose-phosphate aldolase, Dera antibody specific to Mouse Dera .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dera and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dera , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dera in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验VE-1 binding sites (comprising the 9 bp putative interacting sequence and three flanking base pairs) were determined by ELISA and normalized to VE-1 subs. The positions of the potential binding sites relative to the transcription start site
Studies of the Ubiquitin Proteasome System
or other proteasome inhibitor (e.g., lactacystin; Calbiochem or Biomol) Phosphate‐buffered saline (PBS; see recipe ) Nondenaturing Triton X‐100 lysis buffer (see recipe ) containing 1×
“Do‐It‐Yourself” Additional Materials Mouse IgG 1 monoclonal antibody for surface marker of interest Phosphate‐buffered
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