DNA (cytosine-5)-methyltransfe

Mouse DNA (cytosine- 5)- methyltransferase 3- like, DNMT3L ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DNMT3L,DNA (cytosine-5)-methyltransferase 3-like; cytosine-5-methyltransferase 3-like protein; cytosine-5-methyltransferase 3-like protein; DNA (cytosine-5-)-methyltransferase 3-like

Search name

Mouse DNMT3L ELISA KIT ,Mouse DNA (cytosine-5)-methyltransferase 3-like ELISA KIT ,Mouse cytosine-5-methyltransferase 3-like protein ELISA KIT ,Mouse cytosine-5-methyltransferase 3-like protein ELISA KIT ,Mouse DNA (cytosine-5-)-methyltransferase 3-like ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse DNA (cytosine- 5)- methyltransferase 3- like, DNMT3L concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNMT3L. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DNMT3L and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNMT3L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DNMT3L in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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