Cholesterol 24-hydroxylase, Cy
p46a1 ELISA KIT/ Mouse Cholesterol 24-hydroxylase, Cyp46a1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-31955m
- 2026年01月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Cholesterol 24-hydroxylase, Cyp46a1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Cholesterol 24- hydroxylase, Cyp46a1 ELISA KIT
Product Name:Mouse Cholesterol 24- hydroxylase, Cyp46a1 ELISA KIT
Packing:96T
Catalog No.:ELI-31955m
Gene Name:Mouse Cyp46a1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Cyp46a1
Alternative Name:Mouse Cholesterol 24-hydroxylase, Cyp46a1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cholesterol 24- hydroxylase, Cyp46a1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cyp46a1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cholesterol 24- hydroxylase, Cyp46a1 ELISA KIT has been pre-coated with an Mouse Cholesterol 24-hydroxylase, Cyp46a1 antibody specific to Mouse Cyp46a1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyp46a1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyp46a1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyp46a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验for the determination of cholesterol 7α-hydroxylase acttvity based on the conversion by cholesterol oxidase of the primary P450 metabohte 7α-hydroxycholesterol to 7α-hydroxy-4-cholesten-3-one, which can be detected at 254 nm.
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
Mouse Strains and Genetic Nomenclature
Figure 3. This table was captured from a window from the Mouse Phenome Database. It represents the complete set of data for blood cholesterol performed on both male and female mice of 43 different inbred
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