- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-31755m
- 2026年01月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Histone H3.2, Hist1h3b
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Histone H3.2, Hist1h3b ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Hist1h3b,H3-53,H3.2,H3b,H3/l; H3FL; H3 histone family member L; histone 1 H3b; histone H3.1; histone H3/l; histone cluster 1 H3b
Search name
Mouse Hist1h3b ELISA KIT ,Mouse H3-53 ELISA KIT ,Mouse H3.2 ELISA KIT ,Mouse H3b ELISA KIT ,Mouse H3/l ELISA KIT ,Mouse H3FL ELISA KIT ,Mouse H3 histone family member L ELISA KIT ,Mouse histone 1 H3b ELISA KIT ,Mouse histone H3.1 ELISA KIT ,Mouse histone H3/l ELISA KIT ,Mouse histone cluster 1 H3b ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Histone H3.2, Hist1h3b concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hist1h3b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hist1h3b and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hist1h3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
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文献和实验upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
Identifying Differential Histone Modification Sites from ChIPseq Data
a hidden Markov model (HMM) to infer the states of histone modification changes at each genomic location. We evaluated the performance of ChIPDiff by comparing the H3K27me3 modification sites between mouse embryonic stem cell (ESC) and neural progenitor
Chromatin Immunoprecipitation Protocol for Histone Modification
. In this protocol, we will describe the method used in our laboratory. This protocol has been successfully used in experiments with Mouse Embryonic Stem (ES) cells, Embryonic Fibroblasts (MEF), Trophoblast Stem (TS) cells, B-lymphocytes and hepatocytes
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