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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Dead end protein homolog 1, Dnd1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Dead end protein homolog 1, Dnd1 ELISA KIT
Product Name:Mouse Dead end protein homolog 1, Dnd1 ELISA KIT
Packing:96T
Catalog No.:ELI-31726m
Gene Name:Mouse Dnd1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Dnd1
Alternative Name:Mouse Dead end protein homolog 1, Dnd1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dead end protein homolog 1, Dnd1 ELISA KIT allows for the in vitro quantitative determination of Mouse Dnd1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dead end protein homolog 1, Dnd1 ELISA KIT has been pre-coated with an Mouse Dead end protein homolog 1, Dnd1 antibody specific to Mouse Dnd1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dnd1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dnd1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dnd1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验of scientific settings: You have isolated a protein and managed to sequence some amino acids from it. You want to find the corresponding gene! Why not try with degenerate PCR? You have found a human gene and want to clone the homolog gene from e.g
Studies of the Ubiquitin Proteasome System
Basic Protocol 9: Substrate Ubiquitylation by Multisubunit E3s Materials VBC‐Cul2 E3 protein complex expressed and purified using baculoviral expression system ( protocol 45 ) Mouse E1
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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