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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Growth factor receptor-bound protein 10, Grb10
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Growth factor receptor- bound protein 10, GRB10 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GRB10,GRBIR,GRB10 adaptor protein; GRB10 adapter protein; GRB-IR; Grb-10; IRBP; MEG1; RSS; insulin receptor-binding protein Grb-IR; maternally expressed gene 1; growth factor receptor-bound protein 10
Search name
Mouse GRB10 ELISA KIT ,Mouse GRBIR ELISA KIT ,Mouse GRB10 adaptor protein ELISA KIT ,Mouse GRB10 adapter protein ELISA KIT ,Mouse GRB-IR ELISA KIT ,Mouse Grb-10 ELISA KIT ,Mouse IRBP ELISA KIT ,Mouse MEG1 ELISA KIT ,Mouse RSS ELISA KIT ,Mouse insulin receptor-binding protein Grb-IR ELISA KIT ,Mouse maternally expressed gene 1 ELISA KIT ,Mouse growth factor receptor-bound protein 10 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Growth factor receptor- bound protein 10, GRB10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRB10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GRB10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GRB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GRB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验their signal by binding to a fibroblast growth factor receptor (FGFR) which has an intracellular protein tyrosine kinase domain. The tyrosine kinase domain is activated upon FGF binding, resulting in the activation of a transcription factor by means of a signal
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
. Fig. 4 Inhibition of formation of contractile microfilaments by cytochalasin B (CB) leads to a rapid drop of cell index values. BPH-1 cells were pretreated with transforming growth factor-β1 (10 ng/ml) for 68 h and treated with CB (10 μg/ml
PriCells: Primary cells culture with supplement of vascular endothelial growth factor (VEGF)
PriCells: Primary cells culture with supplement of vascular endothelial growth factor (VEGF) Introduction Vascular endothelial growth factor (VEGF) is a signal protein produced by cells that inspires the growth of new blood vessels.
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