Keratin, type II cytoskeletal
2 oral, Krt76 ELISA KIT/ Mouse Keratin, type II cytoskeletal 2 oral, Krt76 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-31150m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Keratin, type II cytoskeletal 2 oral, Krt76
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Keratin, type II cytoskeletal 2 oral, Krt76 ELISA KIT
Product Name:Mouse Keratin, type II cytoskeletal 2 oral, Krt76 ELISA KIT
Packing:96T
Catalog No.:ELI-31150m
Gene Name:Mouse Krt76
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Krt76
Alternative Name:Mouse Keratin, type II cytoskeletal 2 oral, Krt76
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Keratin, type II cytoskeletal 2 oral, Krt76 ELISA KIT allows for the in vitro quantitative determination of Mouse Krt76 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Keratin, type II cytoskeletal 2 oral, Krt76 ELISA KIT has been pre-coated with an Mouse Keratin, type II cytoskeletal 2 oral, Krt76 antibody specific to Mouse Krt76 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Krt76 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Krt76 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Krt76 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics
All eukaryotic cells contain three distinct cytoskeletal networks: microtubules, microfilaments, and intermediate filaments (IF). In epithelial cells in vivo, the IF cytoskeleton is composed of heteropolymers of type I (acidic) and type II
Summary The intermediate filament (IF) cytoskeleton of mammalian epithelia is generated from pairs of type I and type II keratins that are encoded by two large gene families, made up of 54 genes in humans and the mouse
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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