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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Glutathione S-transferase Mu 5, Gstm5
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Glutathione S- transferase Mu 5, Gstm5 ELISA KIT
Product Name:Mouse Glutathione S- transferase Mu 5, Gstm5 ELISA KIT
Packing:96T
Catalog No.:ELI-30838m
Gene Name:Mouse Gstm5
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Gstm5
Alternative Name:Mouse Glutathione S-transferase Mu 5, Gstm5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glutathione S- transferase Mu 5, Gstm5 ELISA KIT allows for the in vitro quantitative determination of Mouse Gstm5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glutathione S- transferase Mu 5, Gstm5 ELISA KIT has been pre-coated with an Mouse Glutathione S-transferase Mu 5, Gstm5 antibody specific to Mouse Gstm5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gstm5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gstm5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gstm5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
lavage. Vet. Clin. Small Anim. 33:69‐88. Blé, F.‐X, Cannet, C., Zurbruegg, S., Karmouty Quintana, H., Frossard, N., Trifilieff, A., and Beckmann, N. 2008. Allergen‐induced lung
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
μl of ice-cold 1 x PBS ,remove 10 μl of these resuspend cells into labeled tubes (for later use in SDS-PAGE analysis). -Note: Except where noted,keep all samples and tubes on ice. Lyse the Cell s using the sonicator equipped
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