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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Peptidyl-tRNA hydrolase 2, mitochondrial, Ptrh2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Peptidyl- tRNA hydrolase 2, mitochondrial, Ptrh2 ELISA KIT
Product Name:Mouse Peptidyl- tRNA hydrolase 2, mitochondrial, Ptrh2 ELISA KIT
Packing:96T
Catalog No.:ELI-30522m
Gene Name:Mouse Ptrh2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ptrh2
Alternative Name:Mouse Peptidyl-tRNA hydrolase 2, mitochondrial, Ptrh2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Peptidyl- tRNA hydrolase 2, mitochondrial, Ptrh2 ELISA KIT allows for the in vitro quantitative determination of Mouse Ptrh2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Peptidyl- tRNA hydrolase 2, mitochondrial, Ptrh2 ELISA KIT has been pre-coated with an Mouse Peptidyl-tRNA hydrolase 2, mitochondrial, Ptrh2 antibody specific to Mouse Ptrh2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ptrh2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ptrh2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ptrh2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
for mitochondrial proteins 117. MITOP http://www.mips.biochem.mpg.de/proj/medgen/mitop/ Mitochondrial proteins, genes and diseases 118. MOsDB http://mips.gsf.de/proj/rice Oryza sativa genome 119. Mouse Genome Database (MGD) http://www.informatics.jax.org
secondary antibody review -- data from 99 publications
ref host class subclass conjugation experiment dilution detail supplier 1 goat Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat Alexa Fluor 546 immunocytochemistry 1:250
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