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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ras-related protein Rab-5B, Rab5b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ras- related protein Rab- 5B, RAB5B ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
RAB5B,RAB5B, member RAS oncogene family
Search name
Mouse RAB5B ELISA KIT ,Mouse RAB5B ELISA KIT ,Mouse member RAS oncogene family ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Ras- related protein Rab- 5B, RAB5B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ras- related protein Rab- 5B, RAB5B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Ras- related protein Rab- 5B, RAB5B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ras- related protein Rab- 5B, RAB5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Ras- related protein Rab- 5B, RAB5B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Rab proteins are small Ras-like GTPases that regulate vesicular trafficking events in the cell. More than 50 Rabs have been described in mammalian cells (1 ), each with a specific subcellular localization reflecting the functional
Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
of monomeric small GTPases of the Ras superfamily, the function of Rab proteins is modulated by guanine nucleotide binding and hydrolysis. To investigate the function of Rab proteins, creating dominant negative or constitutively active mutant forms of a Rab
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人 Dickkopf related protein 1 ( DKK-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 DKK-1 单抗包被于酶标板上,标准品和样品中的 DKK-1与单抗结合,加入生物素化的抗人 DKK-1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入
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